FIGURE 2.
Identification of phosphorylated peptides in IRF3 phosphorylated in vitro with TBK1. A, full-length GST-IRF3 was produced in E. coli and purified on GSTrap HP before elution and removal of the glutathione S-transferase tag with PreScission protease. The purity was examined by SDS-PAGE and staining with Coomassie Blue. B, IRF3 purified from E. coli was phosphorylated in vitro with TBK1 and separated by BN-PAGE prior to immunoblotting (IB) with antibodies recognizing IRF3 phosphorylated on Ser396, Ser386, or total IRF3.