FIGURE 3.

Emitted eGFP fluorescence from various GT-8/+ tissues. A, unstained whole mount confocal microscopy of 2-month GT-8/+ tendon shows incorporation of mutant eGFP-tagged fibrillin-1 into long microfibrils in the longitudinal axis of the tendon. B, unstained 2-month wild-type (+/+) and GT-8/+ skin thin sections show specific incorporation of mutant eGFP-tagged fibrillin-1 into typical microfibril patterns in the dermis and at the dermal-epidermal junction. Scale bar = 50 μm. C, unstained sections of aorta from E16 and P3 heterozygous GT-8 mice revealed green fluorescence, visible at E16, which intensified from E16 to the early postnatal period. Scale bar = 20 μm. D, unstained peripheral blood vessels also accumulated large amounts of fibrillin-1 after birth and demonstrated increasing amounts of green fluorescence in heterozygous mice during the early postnatal period. Scale bar = 50 μm.