FIGURE 5.

Ultrastructure of microfibrils in P0 skin from wild-type and homozygous GT-8 mice. Immunogold labeling of wild-type (+/+) and homozygous (GT-8/GT-8) skin demonstrated the incorporation of truncated fibrillin-1 into microfibrils. Microfibrils in the homozygote were well labeled both with anti-fibrillin-1 (pAb 9543) and with anti-GFP. At P0, anti-fibrillin-2 (pAb 0868) showed only minimal immunogold labeling in both the wild-type and homozygous skin. Although the ultrastructural appearance of the mutant microfibrils was similar to wild-type, the periodicity of pAb 9543 immunogold labeling was not obvious along the lengths of mutant microfibrils. Scale bar = 100 nm.