FIGURE 6.

PDGF-B-induced scratch wound healing in primary mouse embryonic fibroblasts is sensitive to the metalloproteinase inhibitor MM and the EGFR inhibitor AG1478. A, scratch wounds were introduced in confluent cultures of primary wild type mEFs and the cells were then treated with or without PDGF-B (10 ng/ml) or PDGF-B and MM (4 μm) or PDGF-B and the EGFR-inhibitor AG1478 (1 μm) for 10 h. Treatment with PDGF-B significantly increased the number of cells that were present in the scratch-wounded area (between the vertical lines) compared with untreated cells, and this increase was inhibited by MM or AG1478. B, results of three independent experiments were quantified by counting the number of cells that had entered the scratch wound under various conditions, as described under “Experimental Procedures” (n = 3 separate experiments ± S.E.). Following analysis of variance with Bonferroni post hoc analysis, p values were calculated as <0.001 between unstimulated and PDGF-B-treated samples, between PDGF-B and PDGF-B + MM samples, and between PDGF-B and PDGF-B + AG1478 samples (as denoted by asterisks). These results provide evidence for the functional relevance of the metalloproteinase-dependent cross-talk between PDGFRβ and EGFR signaling pathways.