TABLE 2.
Specific activities and kinetic parameters of wild type and mutant forms of S. aureus fRMsr
Enzyme assays were performed using 10 μg of purified proteins as described under “Experimental Procedures.” Values in parenthesis represent the activity relative to wild type. Km and Vmax values were determined by fitting the data to the Michaelis-Menten equation. NA, not assayed.
| Proteins | Specific activity | Km | Vmax |
|---|---|---|---|
| nmol/min/mg protein | μm | nmol/min/mg protein | |
| Wild type | 85 ± 5 (100) | 50 ± 10 | 360 ± 10 |
| C68S | 27 ± 2 (32) | 210 ± 20 | 280 ± 10 |
| C78S | 64 ± 20 (75) | 110 ± 50 | 440 ± 50 |
| C102S | 0 ± 0.3 (0) | NA | NA |
| C68S/C78S | 19 ± 3 (22) | 830 ± 240 | 130 ± 20 |
| C68S/C102S | 0 ± 0.3 (0) | NA | NA |