Skip to main content
. 2010 Jun 7;285(32):25094–25102. doi: 10.1074/jbc.M110.126888

FIGURE 4.

FIGURE 4.

Consequences of SULTR1;2-OASTL interactions on SO42− uptake and OASTL activity. A, SULTR1;2-HA expression in S. cerevisiae cells or in S. cerevisiae cells also expressing OASTL-FLAG (OASTL) or the K46A or H221A mutant of OASTL-FLAG (OASTL K46A or OASTL H221A). Signal strengths were quantified with ImageJ and presented as a ratio of anti-hemagglutinin antibodies to anti-PMA1. B, SO42− uptake by SULTR1;2-HA in the various cells described in A. SO42− uptake by cells grown in high (H) or low (L) SO42− medium was assayed. Error bars are standard deviations (n = 5–13). Lowercase letters a–c indicate the statistically significant differences observed based on Student's t tests between vector control and OASTL co-expression tested under the same sulfur condition (a and b) or between different sulfur conditions with the same transformant (c); p = 6 × 10−5 for a, p = 0.03 for b, and p = 0.056 for c.