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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 1;368(3):614–619. doi: 10.1016/j.bbrc.2008.01.109

Fig. 3.

Fig. 3

Addition of the AK substrates, ATP and AMP, stimulates K-ATP channel activity in human islets. (A) A continuous record of K-ATP channel currents in an inside-out patch held at −60 mV. Unitary current amplitudes are about 4 pA. The record starts with control (Cont.) solution (ATP- and AMP-free) being perfused onto the cytosolic face of the patch. This solution was then exchanged for one containing 30 μM AMP, then 30 μM ATP (zero AMP) then ATP + AMP (both at 30 μM) and finally washout with control solution. Solution applications are indicated by bars above the trace. Values for NPO were 4.40 (Cont.), 3.95 (AMP), 0.96 (ATP), 2.88 (ATP + AMP) and 4.53 (Wash) for this patch. (B) Currents in the presence of ATP are shown on expanded scales at the time indicated below trace A by the bar labeled B. (C) Currents are shown on expanded scales in the presence of ATP + AMP as indicated by bar C marked below trace A. Similar results were obtained in four patches from human β-cells.