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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Biol Inorg Chem. 2010 Mar 12;15(5):717–728. doi: 10.1007/s00775-010-0639-9

Fig. 9.

Fig. 9

Titration of di-Fe(III)–DF3 (78 µM) with NaN3 in 50 mM HEPES, 0.1 M NaCl, pH 7 in a 1 cm path length quartz cuvette. UV– vis spectrum of di-Fe(III)–DF3 upon addition of increasing amounts of NaN3. The inset shows the increase in absorbance at 460 nm as a function of ligand concentration. The curve was obtained from a fit of the data to the Hill equation (see the text)