Figure 10.

a, Schematic of in silico analyses of predicted miRNA binding sites (TargetScan 3.1; www.targetscan.org) shows that three of the ethanol-sensitive miRNAs, miR-153, miR-335, and miR-21 (highlighted by arrows), bind to the 3′ untranslated region of Jag-1 mRNA. b, Bar graph indicates that combined knockdown of miR-21, -153, and miR-335 induces a statistically significant upregulation of Jag-1. Data (CT for Jag-1, relative to CT for cyclophilin-A, expressed as $\frac{\text{1}}{{\text{2}}^{\Delta C{T}_{(Jag{1}_{\text{CyclophilinA}})}}}$) were normalized to the average of the control samples $({\overline{X}}_{\text{control}}(\frac{\text{1}}{{\text{2}}^{\Delta C{T}_{(Jag1-\text{CyclophilinA})}}}))$. c, The bar graph indicates that ethanol exposure (320 mg/dl for 5 d) leads to a significant increase in Jag-1 mRNA expression. Data were expressed as mean ± SEM. Asterisks indicate statistical significance compared with controls; p values are as indicated in the text.