Figure 6.
a, Real-time RT-PCR illustrating significant downregulation of miR-21 after miR-21 knockdown with antisense 2′ O-methyl bases compared with control (nonsense oligonucleotide). The y-axis (expressed as 1/2ΔCT) indicates miRNA expression in cultures, normalized to U6SNR. b, Bar graph depicting miR-335 expression after either miR-335 knockdown alone, or in combination with miR-21 knockdown. miR-335 expression in the combined knockdown of miR-21 and miR-335 was similar to the expression after miR-335 knockdown alone. Data were expressed as mean ± SEM.