Figure 8.

a, LDH cell death assay showed that miR-21 knockdown induced statistically significant cell death, as reported previously in Figure 7. Concurrent exposure to antisense for miR-335 or for miR-335 plus miR-9 completely prevented the cell death induced by transfection with the antisense to miR-21. Data were based on eight independent replicates and expressed as mean ± SEM. Asterisks indicate statistical significance compared with controls; p values are as indicated in the text. b, Labeling of DNA with DAPI reveals the presence of intact nuclei in nonsense controls. c, d, Exposure to antisense anti-miR-21 induced nuclear fragmentation. f, g, Concurrent exposure to anti-miR-335 antisense along with anti-miR-21 antisense prevented cell death induced by anti-miR-21 alone. Photomicrographs were obtained from cells fixed 7 h after transfection. Arrowheads depict the presence of nuclear DNA fragments dispersed within the cytoplasm. Scale bar: (in b) b–g, 25 μm.