Fig. 5.

Microtubules push on the cortex of cells depleted for active Tao-1, generating protrusions. (A-D) Stably expressed EB1-GFP visualised in control S2 cells (A), in cells overexpressing (OE) K56A RFP-Tao-1 (B), in Tao-1 RNAi cells (C) and in cells following treatment with latrunculin B (D). Times between frames are indicated in seconds. Scale bars: 2.5 μm. (E) The average (mean) lifetimes of GFP-EB1 comets pausing at the cell cortex before undergoing catastrophe determined by analysing frames taken every 2 seconds in movies of control, RFP-Tao-1- and K56A RFP Tao-1-expressing cells. Error bars represent s.d.