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. 2010 Jul 27;123(16):2792–2802. doi: 10.1242/jcs.065268

Fig. 5.

Fig. 5.

Progenitor marker mRNAs are enriched in LCM-excised duct epithelium of young regenerating foci compared with mature ducts (CPDs). (A) LCM enables specific excision of duct epithelium from young regenerating foci (yellow-dotted lines); from more mature foci, the RNA quality is compromised by the exocrine enzymes. (B) By semi-quantitative RT-PCR, pancreatic progenitor markers show greater expression in LCM-captured ducts from regenerating foci than in mature control ducts (CPD) from unoperated and sham-operated rats. Sample numbers given in parentheses. Ribosomal proteins L32 (Rpl32) and 18S were used as internal control. (C) Fold change of Pdx1, Sox9, Tcf2 and Hnf6 mRNA in LCM-captured ducts was compared with mature (sham-operated CPD) ducts using real time RT-PCR analysis, with Rpl32 as reference gene. n=3 samples of pooled LCM-captured ducts (each from three to four Px +3-4 day rats). Data presented as mean ± s.e.m.