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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Dev Biol. 2010 Jun 4;344(2):745–757. doi: 10.1016/j.ydbio.2010.05.506

Figure 10. PAR-3 phosphorylation at S863 in par-3(it71) embryos.

Figure 10

(A) in vitro PKC-3 kinase assay with a portion of PAR-3(aa 678-932) and the mutated variants PAR-3S863A::GFP, PAR-3S863E::GFP and PAR-3S863AS865A::GFP as substrate. PKC-3K266A, the kinase-dead form of PKC-3, was used as negative control. (B–E) Anti-PAR-3 antibody stained par-3(it71) embryos that express PAR-3::GFP (B), PAR-3S863A::GFP (C), PAR-3S863E::GFP (D) and PAR-3S863AS865A::GFP (E).