(A) in vitro PKC-3 kinase assay with a portion of PAR-3(aa 678-932) and the mutated variants PAR-3S863A::GFP, PAR-3S863E::GFP and PAR-3S863AS865A::GFP as substrate. PKC-3K266A, the kinase-dead form of PKC-3, was used as negative control. (B–E) Anti-PAR-3 antibody stained par-3(it71) embryos that express PAR-3::GFP (B), PAR-3S863A::GFP (C), PAR-3S863E::GFP (D) and PAR-3S863AS865A::GFP (E).