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. 2010 Jul 12;17(1):56. doi: 10.1186/1423-0127-17-56

Figure 7.

Figure 7

The involvement of Akt and ERK activation in FN or LAM-induced enhancement of IPC differentiation. (A) MSCs were transduced with scrambled (-) or siRNA against ERK (siERK) and Akt (siAkt) and induced for IPC differentiation with FN or LAM, and western blotting was done for stage IV cells. Quantification of Akt (B) and ERK (C) phosphorylation after transduction with siERK and siAkt. Quantitative RT-PCR for (D) insulin and (E) Glut2 expression shows transduction with either siERK or siAkt increases the expression of insulin and Glut2, and transduction with both siERK and siAkt decreases the expression of insulin and Glut2. (mean ± S.D.; *p < 0.05 and **p < 0.01 compared with the scrambled as determined by the student's t test.).