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Schematic structure of the NPHS2-promoter/ADA transgene. The transgene construct carried a 2.6-kb DNA fragment located upstream the translated region of the human NPHS2 (hatched) gene followed by the mouse ADA cDNA (dotted). A HA-tag was introduced at the 3' end of ADA (black), just before the stop codon. Finally, the poly-adenylation (grey) sequence corresponds to the SV40 Late poly(A) from pCi vector. Arrows indicate the location of primers used for PCR genotyping of mice.
