Table 1.
Thirty nine selected 2-DE spots (proteins) from the hemocytes of Vibrio harveyi-challenged Penaeus monodon, resolved by 2-DE and identified by LC-nano ESI-MS/MS.
| Spot no. | Predicted MW (Da) | Predicted pI | Protein hit | Mowse Score/no. of match peptides |
Accession no. | Fold change* | |
|---|---|---|---|---|---|---|---|
| 24 h | 48 h | ||||||
| Up-regulated protein spots | |||||||
| 33 | 78700 | 6.05 | prophenoloxidase 2 [Litopenaeus vannamei] | 71/2 | ABQ45957 | U | U |
| 30 | 41822 | 5.11 | actin 2 [Penaeus monodon] | 240/7 | AAC78682 | U | U |
| 3 | 74934 | 5.27 | hemocyanin [Litopenaeus vannamei] | 99/4 | CAA57880 | -1.35 | +6.88 |
| 16 | unknown | -2.92 | +4.11 | ||||
| 35 | 40087 | 6.05 | arginine kinase [Penaeus monodon] | 485/13 | AAO15713 | +1.85 | +4.09 |
| 29 | 40087 | 6.05 | arginine kinase [Penaeus monodon] | 704/32 | AAO15713 | +2.33 | +3.16 |
| 2 | 17142 | 6.74 | twinstar [Drosophila melanogaster] | 150/8 | NP_477034 | +1.88 | +1.20 |
| 37 | 49283 | 4.92 | tubulin alpha chain [Oncorhynchus keta] | 94/3 | P30436 | -1.38 | +1.88 |
| 4 | 52810 | 5.08 | serine proteinase-like protein [Penaeus monodon] | 119/5 | ABD62888 | +1.69 | -1.25 |
| 32 | 37134 | 6.73 | transaldolase [Bombyx mori] | 110/1 | NP_001040544 | +1.60 | +1.58 |
| Down-regulated protein spots | |||||||
| 20 | 32431 | 5.04 | alpha-2-macroglobulin [Penaeus monodon] | 59/1 | AAX24130 | D | D |
| 9 | 16671 | 4.04 | calmodulin [Patinopecten sp.] | 54/3 | 0711223A | D | -13.89 |
| 19 | 29054 | 4.65 | 14-3-3 protein epsilon [Danio rerio] | 114/5 | NP_997770 | D | +1.12 |
| 12 | unknown | -1.08 | -3.75 | ||||
| 14 | 96846 | 4.73 | karyopherin (importin) beta [Nematostella vectensis] | 109/3 | XP_001636221 | -8.14 | -3.21 |
| 8 | unknown | -3.42 | -2.70 | ||||
| 36 | 78700 | 6.05 | prophenoloxidase 2 [Litopenaeus vannamei] | 119/3 | ABQ45957 | -3.49 | -2.64 |
| 11 | 78700 | 6.05 | prophenoloxidase 2 [Litopenaeus vannamei] | 127/3 | ABQ45957 | -2.31 | -2.87 |
| 7 | unknown | -1.59 | -2.73 | ||||
| 13 | 24283 | 6.52 | GTP-binding nuclear protein Ran (GTPase Ran) (Ras-like protein TC4) [Brugia malayi] | 104/2 | P38542 | -1.52 | -2.75 |
| 27 | Unknown | -1.35 | -2.39 | ||||
| 6 | 52810 | 5.08 | serine proteinase-like protein [Penaeus monodon] | 84/2 | ABD62888 | -1.37 | -2.42 |
| 10 | 78426 | 5.83 | Prophenoloxidase-1 [Penaeus monodon] | 973/20 | AAM77689 | -1.88 | -2.32 |
| 31 | 56040 | 5.10 | ATP synthase subunit beta, mitochondrial precursor [Hemicentrotus pulcherrimus] | 411/7 | Q25117 | -2.06 | -1.17 |
| 5 | 52810 | 5.08 | serine proteinase-like protein [Penaeus monodon] | 119/5 | ABD62888 | -1.92 | -1.48 |
| 15 | 83278 | 4.89 | heat shock protein 90 [Metapenaeus ensis] | 484/17 | ABR66910 | -1.17 | -1.59 |
| 25 | Unknown | -1.40 | -1.62 | ||||
| Constant protein spots | |||||||
| 38 | 28203 | 5.89 | thymosin beta-11 [Penaeus monodon] | 419/10 | BI018085 | +1.41 | +0.98 |
| 34 | 46851 | 4.41 | calreticulin [Gallus gallus] | 82/3 | AAS49610 | -1.17 | +1.48 |
| 28 | 31358 | 5.42 | cytosolic manganese superoxide dismutase [Penaeus monodon] | 421/12 | AAW50395 | +1.18 | -1.50 |
| 26 | 40087 | 6.05 | arginine kinase [Penaeus monodon] | 103/3 | AAO15713 | +1.03 | +1.16 |
| 24 | 47235 | 6.18 | phosphopyruvatehydratase [Penaeus monodon] | 452/9 | AAC78141 | +1.06 | +1.01 |
| 17 | 32574 | 4.78 | hypothetical protein LOC550536 [Danio rerio] | 58/3 | NP_001017838 | +1.24 | +1.12 |
| 18 | 32420 | 4.70 | tropomyosin [Locusta migratoria] | 305/17 | P31816 | +1.47 | +1.06 |
| 1 | 41841 | 5.30 | beta-actin [Litopenaeus vannamei] | 1083/80 | AAG16253 | +1.46 | +1.32 |
| 39 | Unknown | +1.12 | +1.21 | ||||
| 21 | Unknown | -1.07 | -1.36 | ||||
| 22 | Unknown | +1.21 | +1.14 | ||||
| 23 | Unknown | -1.24 | +1.19 | ||||
* indicates the decrease in the spot intensity at a given time point after V. harveyi infection compared to that of normal shrimp.
+ indicates the increase in the spot intensity at a given time point after V. harveyi infection compared to that of normal shrimp.
U indicates the up-regulated protein spot detected only in the protein extracts of infected animals.
D indicates the down-regulated protein spot detected only in the protein extracts of normal animals.