Figure 6. CDDO-ethyl amide (CDDO-EA) and CDDO-trifluoroethyl amide (CDDO-TFEA) upregulated expression of genes specifically involved in the Nrf2/ARE pathway in N171-82Q mice.

mRNA levels of HO1 in striatum of (A) CDDO-EA treated and (B) CDDO-TFEA treated N171-82Q mice. Data were expressed as means and standard errors. HO1 gene expression was significantly increased in striatum of N171-82Q mice treated with CDDO-EA (p=0.04 at 200mg/kg diet) and CDDO-TFEA (p=0.009 at 200mg/kg diet, p=0.03 at 400mg/kg diet) compared to N171-82Q mice fed control diet.

mRNA levels of (C) GST3a (D) NQO1 and (E) HO1 in skeletal muscle of CDDO-TFEA treated N171-82Q mice. Data were expressed as means and standard errors. mRNA level of GST3a (p=0.007 at 100mg/kg diet, p=0.0001 at 200mg/kg diet, p=0.002 at 400mg/kg diet), NQO1 (p=0.04 at 200mg/kg diet, p=0.02 at 400mg/kg diet) and HO1 (p=0.02 at 200mg/kg diet, p=0.03 at 400mg/kg diet) were significantly increased compared to mice fed control diet.

mRNA levels of (F) GST3a and (G) NQO1 in brown adipose tissue of CDDO-EA treated N171-82Q mice. Data were expressed as means and standard errors. GST3a gene expression was significantly increased in brown adipose tissue of CDDO-EA treated mice compared to control (p=0.005 at 200mg/kg diet). NQO1 gene expression was also significantly increased in brown adipose tissue of CDDO-EA treated mice compared to control (p=0.0002 at 200mg/kg diet).

mRNA levels of (H) GST3a and (I) NQO1 in brown adipose tissue of CDDO-TFEA treated N171-82Q mice. Data were expressed as means and standard errors. Both GST3a gene expression (p=0.003 at 100mg/kg diet, p<0.0001 at 200mg/kg diet, p<0.0001 at 400mg/kg diet) and NQO1 gene expression (p=0.005 at 100mg/kg diet, p=0.03 at 200mg/kg diet, p=0.005 at 400mg/kg diet) were significantly increased in CDDO-TFEA treated mice.