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IL-4 up-regulates mRNA and protein expression of MCP-1 in vivo. Mice were administered a single intraperitoneal injection of either PBS (control) or 100 µg/kg of IL-4, and exposed for 4, 8, and 24 h. The mRNA and protein expression levels of MCP-1 in mouse aortas (a, b) and blood plasma (c) were determined by real-time RT-PCR (a) and ELISA (b, c). Values represent mean ± SEM (n = 4). *p < 0.05 versus control
