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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Free Radic Biol Med. 2010 Apr 18;49(2):268–274. doi: 10.1016/j.freeradbiomed.2010.04.012

Figure 3. Oxygen is required for the L-cysteine-mediated decomposition of the protein-bound DNICs in the starved E. coli cells.

Figure 3

A), the NO-exposed starved E. coli cells (spectrum 1) were incubated at 37°C for 60 min in the M9 minimal medium (without glucose) supplemented with L-cysteine (1 mM) under aerobic (spectrum 2) or anaerobic (spectrum 3) conditions. B), the cell extracts prepared from the NO-exposed starved E. coli cells (spectrum 1) were incubated at 37°C for 30 min with L-cysteine (1 mM) under aerobic (spectrum 2) or anaerobic (spectrum 3) conditions. C), kinetics of the L-cysteine-mediated decomposition of the protein-bound DNICs in the cell extracts under aerobic and anaerobic conditions. The cell extracts prepared from the NO-exposed starved E. coli cells were incubated with L-cysteine (1 mM) at 37°C under aerobic (open circles) or anaerobic (open squares) conditions. The amplitudes of the EPR signal at g = 2.04 of the protein-bound DNICs were plotted as a function of incubation time.