Skip to main content
. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Free Radic Biol Med. 2010 Apr 18;49(2):268–274. doi: 10.1016/j.freeradbiomed.2010.04.012

Figure 5. Role of superoxide and hydrogen peroxide in the L-cysteine-mediated decomposition of the protein-bound DNICs.

Figure 5

Purified aconitase B-bound DNIC (5 µM) (spectrum 1) was incubated with L-cysteine (1 mM) (spectrum 2), or L-cysteine (1 mM) with superoxide dismutase (200 units/ml) (spectrum 3), L-cysteine (1 mM) with catalase (200 units/ml) (spectrum 4), or L-cysteine (1 mM) with superoxide dismutase (200 units/ml) and catalase (200 units/ml) (spectrum 5). Spectrum 6), purified aconitase B-bound DNIC (5 µM) was incubated with superoxide dismutase (200 units/ml) and catalase (200 units/ml) only. All samples were incubated at 37°C for 30 min under aerobic conditions.