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. 2010 Aug;11(8):608–617. doi: 10.1631/jzus.B1001007

Fig. 3.

Fig. 3

Fig. 3

Expression of endogenous Hsp90 and membrane receptors of ErbB2 and TLR-4

(a) Western blot was run to detect the expression of endogenous Hsp90 on MSCs under normoxia and serum-contained (SC) conditions (Lane 1), hypoxia and serum deprivation (SD) (Lane 2), and preconditioning with exogenous rhHsp90α (10 μmol/L) (Lane 3). Representative bands were shown in the top panel. Hypoxia and SD induced endogenous Hsp90 expression in MSCs compared to normoxia and SC (** P<0.01). Exogenous rhHsp90α preconditioning did not change the Hsp90 expression compared to MSCs without preconditioning (* P>0.05, bottom panel); (b) Cell ErbB2 and TLR-4 receptors were analyzed by quantitative RT-PCR. Hypoxia and SD led to increased expression of ErbB2 and TLR-4 mRNA on MSCs, compared to normoxia and SC (** P<0.01). Expression of ErbB2 and TLR-4 mRNA decreased on MSCs preconditioned with exogenous rhHsp90α, compared to MSCs without preconditioning (## P<0.01)