Fig. 4.

Effect of exogenous rhHsp90α preconditioning on PI3K/Akt and ERK1/2 pathways

Representative bands of the interested proteins are shown on the top panel. Compared to normoxia and serum-contained (SC) conditions (Lane 1), hypoxia and serum-deprivation (SD) (Lane 2) resulted in a 3.0-fold decrease of p-Akt/Akt and p-ERK/ERK expression on MSCs, which was reversed by exogenous rhHsp90α preconditioning (^** P<0.01, respectively). MSCs incubated with 17-AAG (40 nmol/L) (Lane 4), an Hsp90 inhibitor, reduced p-Akt/Akt and p-ERK/ERK expression of MSCs preconditioning with exogenous rhHsp90α (10 μmol/L) (Lane 3) (^# P<0.05; ^## P<0.01)