Fig. 5.

Effect of exogenous rhHsp90α preconditioning on the apoptotic proteins

Representative bands of the interested proteins are shown on the top pannel. Compared to normoxia and serum-contained (SC) (Lane 1), hypoxia and serum deprivation (SD) (Lane 2) decreased the expression of Bcl-2 and Bcl-xL proteins, and increased the expression of Bax and cleaved caspase-3 proteins in MSCs (^** P<0.01, respectively). Preconditioning MSCs with exogenous rhHsp90α (10 μmol/L) (Lane 3) elevated the expression of Bcl-2 and Bcl-xL proteins, and alleviated the expression of Bax and cleaved caspase-3 proteins (^## P<0.01, respectively), which were reversed by 17-AAG (an Hsp90 inhibitor, 40 nmol/L) (Lane 4), wortmannin (a PI3K inhibitor, 0.2 μmol/L) (Lane 5) and U0126 (an ERK1/2 inhibitor, 10 μmol/L) (Lane 6); ^** P<0.01 respectively. β-actin as the internal standard