Figure 1.

Myd88^−/− mice develop colonic polyps after AOM/DSS administration. Cohorts of 8–10 WT and Myd88^−/− mice per group were injected i.v. with AOM on day 0 followed by two DSS cycles administered in drinking water. At the completion of the first DSS cycle, mice were monitored for bleeding (A) and diarrhea (B). 60 d after AOM administration, hematocrits were measured (C), mice were euthanized and colons were resected and measured (D), and macroscopic polyps were counted (E). Colon sections were fixed in formalin and stained with hematoxylin-eosin. Sections were analyzed for inflammation degree (F), extent of ulceration (G), and squamous metaplasia (H). The data shown correspond to a representative experiment out of four performed. Data represent means ± SE (and in some panels also individual mice results). *, P < 0.05; **, P < 0.01; ***, P < 0.001.