Figure 3.

AOM/DSS treatment enhances mucosal expression of COX-2 and phospho-STAT3 in Myd88^−/− mice. Cohorts of 5–10 mice were subjected to treatment as indicated in Fig. 2. At day 3 of DSS administration, colons were resected and fixed in formalin or snap frozen for protein extraction. Tissue sections were stained for COX-2 and pSTAT3. Photomicrographs of representative sections from the respective groups are shown at 200× (A). Protein extracts were analyzed by Western blotting for COX-2, STAT3, and pSTAT3 expression. Gapdh was used as a control (B). The data shown in A and B correspond to a representative experiment out of two performed.