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. 2010 Aug 2;207(8):1791–1804. doi: 10.1084/jem.20092454

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© 2010 Thompson et al.

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Figure 4. — Endogenous lymphocytes are not required for naive T cell infiltration into tumors or their in situ activation. C57BL/6 mice or RAG2^−/− mice bearing s.c. tumors received Thy1-mismatched OT-I T cells. 24 h later, lymphocytes from tumors, LN, and spleen were harvested and stained for flow cytometry. Plots are gated on Thy1.1⁺ CD8⁺ lymphocytes, and numbers indicate the percentage of OT-I cells that are single positive for CD69 or CD25, or positive for both markers. Axes on plots are displayed in Logicle scale. Results represent two independent experiments with three mice each per group.