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. 2010 Aug 2;207(8):1727–1743. doi: 10.1084/jem.20100276

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© 2010 Sultana et al.

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Figure 5. — A. phagocytophilum–induced phosphorylated/G-actin accumulates in tick cell nuclei. (A) Nuclear extracts were prepared from uninfected (UI) and A. phagocytophilum–infected (I) cells and subjected to immunoprecipitation (IP) and immunoblot (IB) with the indicated antibodies. Immunoblots for RNAPII and TBP served as loading control. Three independent experiments yielded similar results. (B) Confocal microscopy showing G-actin (red) and F-actin (green) in A. phagocytophilum–infected (I) or uninfected (UI) tick cells. Bar, 10 µM. (C and D) Uninfected and infected tick cells were stained with DNase I for G-actin (red; C) or anti-phosphotyrosine (red; D) and anti RNAPII (green) and TOPRO-3 (blue). Representative images from three independent experiments are shown. Bars, 20 µm.