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O-Digitoxoside 1 and MeON-digitoxoside 4 activate caspase-9, not caspase-8, in NCI-H460 non-small cell lung cancer cells. Cell lysates were incubated in fluorometric substrates for either caspase-8 (IETD-AFC) or caspase-9 (LEHD-AFC); samples were then read using a fluorescence plate reader. Ntx = no treatment. ZV = pan-caspase inhibitor ZVAD-FMK.
