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. 2010 Jul;15(7):689–698. doi: 10.1111/j.1365-2443.2010.01413.x

Figure 1.

Figure 1

Hes1 protein expression in Hes1-sustained embryonic stem (ES) cells. (A) The structure for sustained Hes1 expression. The Hes1 cDNA with the IRES-EGFP sequence was knocked-in into the Rosa26 locus, so that Hes1 and EGFP were constitutively expressed from the Rosa26 promoter (Kobayashi et al. 2009). (B) Hes1 and Oct3/4 expression in the wild-type (WT) and Hes1-sustained (R5, R6) ES cell lines. Actin is a loading control. (C) Immunostaining of Hes1 (red) with DAPI staining (blue) in ES cell lines.