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Hes1 regulates expression of Notch signaling and cell cycle factors during differentiation. (A) mRNA levels of Notch signaling genes, Dll1, Jag1, Hes5 and Notch1. (B) Protein levels of Notch1 receptor, Notch intracellular domain (NICD), p57, Oct3/4 and Hes1 in the wild-type cells (WT; lanes 1, 4, 7 and 10) and in Hes1-sustained cells (R5; lanes 2, 5, 8 and 11, R6; lanes 3, 6, 9 and 12) under a neural differentiation condition.
