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. 2010 May 11;63(1):141–154. doi: 10.1111/j.1365-313X.2010.04228.x

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Journal compilation © 2010 Blackwell Publishing Ltd and the Society for Experimental Biology

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Complementation of rhizobial infection phenotypes of non-nodulating mutants by CCaMK^T265D transformation. (a–h) Transformed plants were inoculated with DsRed-labeled Mesorhizobium loti. (a, c) Mature nodules formed on the roots of ccamk-3/CCaMK^T265D (ccamk/TD) and symrk-3/CCaMK^T265D (symrk-3/TD) after 4 weeks of inoculation. Scale bars are 1 mm. (b, d, g) Root hairs of ccamk/TD, symrk-3/TD and castor-4/CCaMK^T265D (castor/TD) 2 weeks after inoculation, shown as merged images of bright-field and fluorescence images (DsRed). Infection threads can be seen inside the curled root hairs. Scale bars are 100 μm. (e) A mature nodule section of symrk-3/TD stained with toluidine blue. The nodule was filled with differentiated bacteroids. Scale bar is 20 μm. (f, h) Nodule primordia with rhizobial infection on the roots of symrk-7/CCaMK^T265D (symrk-7/TD) and pollux-2/CCaMK^T265D (pollux/TD), shown as merged images of bright-field and fluorescence images (DsRed). Scale bars are 200 μm.