Table 1.
Induction of spontaneous nodulation and restoration of symbiotic defective phenotypes of non-nodulating mutants, transformed with wt-CCaMK (TT) or CCaMKT265D (TD) constructs
| Phenotypes |
||||
|---|---|---|---|---|
| Lotus lines | CCaMK construct | SpNa | Nodb | AMc |
| Gifu (B-129) | TT | 0/24 | 22/23 | 33/33 |
| Gifu (B-129) | TD | 29/43 | 21/21 | 25/25 |
| ccamk-3 | TT | 0/19 | 31/32 | 24/26 |
| ccamk-3 | TD | 57/77 | 32/35 | 21/27 |
| nfr1-4 | TT | 0/91 | 0/29 | nt |
| nfr1-4 | TD | 33/93 | 19/38d | nt |
| nfr5-2 | TT | 0/27 | 0/30 | nt |
| nfr5-2 | TD | 22/32 | 19/33d | nt |
| symrk-3 | TT | 0/5 | 0/20 | nt |
| symrk-3 | TD | 12/18 | 27/42 | nt |
| symrk-7 | TT | 0/36 | 0/40 | 0/24 |
| symrk-7 | TD | 23/54 | 37/41 | 37/38 |
| castor-4 | TT | 0/51 | 0/54 | 0/19 |
| castor-4 | TD | 51/67 | 58/67 | 19/25 |
| pollux-2 | TT | 0/58 | 0/46 | 0/16 |
| pollux-2 | TD | 44/66 | 46/52 | 14/17 |
| nup85-3 | TT | 0/38 | 0/41 | 0/20 |
| nup85-3 | TD | 25/26 | 41/46 | 28/31 |
| cyclops-4 | TT | –e | 15/15f | 0/9 |
| cyclops-4 | TD | –e | 23/27f | 4/17g |
| nsp2-1 | TT | 0/72 | 0/51 | nt |
| nsp2-1 | TD | 0/96 | 0/59 | nt |
| nin-2 | TT | 0/25 | 0/32 | nt |
| nin-2 | TD | 0/36 | 0/30 | nt |
| hit1-1 | TT | 0/64 | 6/22h | nt |
| hit1-1 | TD | 0/73 | 9/50h | nt |
Spontaneous nodulation in the absence of Mesorhizobium loti (scored at 6 weeks after transplantation).
Nodule formation under M. loti inoculation.
Mycorrhization with arbuscule formation.
Empty nodule without rhizobial invasion.
Previously reported by Yano et al. (2008).
Bump-like structure without rhizobial invasion.
Formation of few arbuscules.
Infected nodule with abnormal shape.
nt, not tested.
Number of plants with the phenotypes describe above (a–h) per number of transformed plants are listed. Infection phenotypes were examined 4 weeks after inoculation. Data were compiled from more than two independent experiments.