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. 2010 Jun 15;77(3):658–671. doi: 10.1111/j.1365-2958.2010.07236.x

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© 2010 Blackwell Publishing Ltd

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Fig. 2 — Rescue of RNAi growth defect by expression of LmPTR1. A. Cumulative growth of WT (open circles) non-induced ^oeRNAi cells (open inverted triangles) and tetracycline-induced ^oeRNAi cells (closed triangles). B. Western blot analysis demonstrating knockdown of TbPTR1 in ^oeRNAi cells. Non-induced (-) and 48 h tetracycline induced (+) cells were probed sequentially with anti-TbPTR1 and anti-LmPTR1. A 55 kDa non-specific band was detected with anti-TbPTR1, which was not effect by the knockdown of PTR1 and serves as an internal loading control (top panel), while LmPTR1 is present in both (bottom panel).