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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Hypertension. 2010 May 3;55(6):1461–1467. doi: 10.1161/HYPERTENSIONAHA.110.150029

Figure 2. Effect of Ang II and AA on CYP1B1 activity.

Figure 2

To measure the activity of CYP1B1, VSMCs were transduced with Ad-Sc CYP1B1 shRNA (Ad-Sc), Ad CYP1B1 shRNA (Ad-shRNA), or Ad-EV for 48 hours or pretreated with TMS (100 nmol/L) or its vehicle (Veh) for 30 minutes before adding Ang II (200 nmol/L) or AA (30 μmol/L) or their vehicle for 20 minutes. CYP1B1 activity was measured using luciferin detection agent (LDR) and luminescence was measured as described in “Methods”. Values are means ± S.E. * denotes a value significantly different from the corresponding value obtained in cells transduced with Ad-Sc (p<0.05). *denotes a value significantly different from the corresponding value obtained in the presence of Veh of TMS (p<0.05).