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rmSEB is not a competitive inhibitor of native SEB for pig leukocytes. Pig splenic leukocytes were isolated and cocultured in the presence of 1.0 μg/ml of native SEB (nSEB) with the indicated concentrations of rmSEB. After 40 h of culture, supernatants were taken, and porcine gamma interferon (IFN gamma) secretion was determined using an ELISA. Results are presented as mean values (±SEM) for triplicate determinations.
