FIG. 1.

Interaction of YopM alleles with RSK1 and PRK2. (A) Representation of the YopM constructs used in this study. The black numbered boxes represent the LRRs, while the N-terminal dark gray boxes are the α-helical domains defined in reference 17. The C-terminal disordered region is represented as a pale gray box. (B and C) GST-YopM pulldown assays. GST alone or the GST-YopM constructs represented in panel A were purified and added in equimolar amounts to GST-Bind Sepharose beads. The protein-bound beads were then added to lysates of THP-1 monocytic cells, and the proteins were allowed to bind overnight before being removed and washed. Samples were processed for SDS-PAGE and Western blotting using antibodies against PRK2 or RSK1. Lane 1 shows a sample of the starting lysate. S, 10%, by volume, of the postbinding supernatant; B, GST-Bind bead-bound proteins; WT, wild type. Positions of molecular mass markers are shown on the left.