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. 2010 Jun 14;78(8):3637–3646. doi: 10.1128/IAI.00296-10

FIG. 3.

FIG. 3.

Decreased autolysin activity of the pknB mutant. (A) Bacteriolytic activities of secreted autolysins in the supernatants of stationary phase cultures, using heat-killed USA300 cells as the substrate. (B) Zymographic analysis of autolytic activity of the pknB mutant of USA300 using either M. lysodeikticus (top panel) or S. aureus 4220 (bottom panel) as the substrate. Note the decrease in the activity of the proenzyme for major autolysin Atl (130 kDa) and the processed autolysin (110 kDa; arrow) while the activities of the glucosaminidase (55 kDa) and amidase (63 kDa) cleaved from Atl are relatively unchanged. Numbers underneath the gel panels indicate the relative band intensities as determined by ImageJ.