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. 2010 Jun 1;78(8):3378–3391. doi: 10.1128/IAI.00342-10

FIG. 2.

FIG. 2.

Stx1 induces TRAIL and DR5 expression by macrophage-like THP-1 cells. (A) Macrophage-like THP-1 cells were treated with the indicated amounts of Stx1 for 24 h. Cell-free supernatants and cellular lysates were collected and analyzed by ELISA specific for human TRAIL production. C, control (untreated) cells. The data shown are means ± SEM from at least three independent experiments. *, significant difference (P < 0.05) between Stx1-treated cells and control cells. (B) Analysis of DR5 mRNA expression by RT-PCR using total RNA extracted from macrophage-like THP-1 cells treated with Stx1 for 0 to 24 h (upper panel), or Stx1 or Stx1A− for 4 h (lower panel). Primers for amplification of β-actin were used as a control. The data shown are characteristic of three independent experiments. (C) Analysis of DR5 protein expression by Western blotting (WB) using cell lysates prepared from macrophage-like THP-1 cells treated with Stx1 or thapsigargin (Tg) for 0 to 16 h, with Stx1A− or Stx1 B subunits for 0 to 16 h, or with Tg for 4 h. Antibodies directed against β-actin were used as a loading control. The data shown are characteristic of three independent experiments.