Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristic | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | F−ompT hsdSB(rB− mB−) dcm gal (DE3) | Novagen (Madison, WI) |
| Y. pestis | ||
| KIM5 | pCD1+ (LCR+) pgm (Pgm−) | 23 |
| KIM6 | KIM5 pCD1− (LCR−) | 23 |
| DSY24 | KIM5 Δcaf::blaM | This study |
| DSY39 | KIM5 Δcaf::blaM Δpsa::aphA | This study |
| Plasmids | ||
| pCS267 | pBR322-psaABC | 37 |
| pCS274 | pET16-caf1M-caf1A-caf1 | 37 |
| pDMS167 | pBR322fas (987P+) | 59 |
