TABLE 2.
Oligonucleotide primers used in this study
| Primer name | Oligonucleotide (5′-3′)a |
|---|---|
| up-luxS-f-BamHI | GCGggatccTTGTTCGACTGCTTTTCTGA |
| up-luxS-r-SalI | GCGgtcgacGAATGTTGAAAGTTTCAATT |
| down-luxS-f-PstI | GCGctgcagCGCTTATGATTTGTTGCATTATAA |
| down-luxS-r-HindIII | GCGaagcttTATAGCTGGCCCGTCAAGTC |
| up-kdpDE-f-BamHI | GCGggatccTCTACTCTTGACGATTGCAC |
| up-kdpDE-r-SalI | GCGgtcgacATCTAAATATT |
| down-kdpDE-f-HindIII | ATTCaagcttTAGATAACGG |
| down-kdpDE-r-XbaI | GCGtctagaCACCAAATGTGGTGAGTATA |
| DEL-kdpE-f-XbaI | GCGtctagaTCAATTTGATTGAAAATGCA |
| DEL-kdpE-r-BamHI | GCGggatccAACGCATTTCTACAGAGTTG |
| Em-f-NdeI | GCGcatatgGATACAAATTCCCCGTAGGC |
| Em-f-PstI | GCGctgcagGATACAAATTCCCCGTAGGC |
| Em-f-HindIII | GCGaagcttGATACAAATTCCCCGTAGGC |
| Em-r-HindIII | GCGaagcttGAAATAGATTTAAAAATTTCGC |
| Em-r-SalI | GCGgtcgacGAAATAGATTTAAAAATTTCGC |
| c-luxS-f-HindIII | GCGaagcttAAAGCATCGTATTCTGCTAA |
| c-luxS-r-EcoRI | GCGggatccTGAAAAATACAATCAATCTA |
| c-kdpDE-f-EcoRI | GCGgaattcCATTGTTAGAAACAAAATTATTTC |
| c-kdpDE-r-BamHI | GCGggatccCCACCAAATGTGGTGAGTAT |
| p-kdpDE-f-EcoRI | GCGgaattcATATTTTCAAATCTAGTAAATATTA |
| p-kdpDE-r-SmaI | GCGcccgggAACCTTCACCTCGATAGCAA |
| g-kdpE-f-SmaI | GCGcccgggATGCAATCTAAAATATTGATAATT |
| g-kdpE-r-BamHI | GCGggatccTTATTTCTCTTTCCACTGCA |
| M-kdpEAsp-f | TTAGGTTTACCAGATAAAGATGGAT |
| M-kdpEAsp-r | TAATAAAATGACATCTGGTTTATCA |
| p-cap-f | TCTATCTGATAATAATCATCTAACT |
| p-cap-r | TATTTACCTCCCTTAAAAAT |
| g-kdpE-f-NcoI | GCGccatggTGCAATCTAAAATATTGATAATTGAAG |
| g-kdpE-r-XhoI | GCGctcgagTTTAATATCATCTCATAAGT |
| rt-16S-f | CGTGGAGGGTCATTGGA |
| rt-16S-r | CGTTTACGGCGTGGACTA |
| rt-capA-f | CAGTTAAAGTCGCACCAA |
| rt-capA-r | GAACCCAATACAGGCAAT |
| rt-capC-f | CGTCATTAGCGGGTATTT |
| rt-capC-r | TCTTGTTGTGGCATTCGT |
| rt-capE-f | TTTCCAGTTGAGGCAGTG |
| rt-capE-r | TTCTTGATTTGGCTACGA |
| rt-capG-f | TTGAAGTTCCCTGGTGTC |
| rt-capG-r | CATCGGTTCGTTATTGTT |
| rt-capI-f | AAGGAAGCGAATGTTAT |
| rt-capI-r | AGCTGATGAAGCAATAA |
| rt-capK-f | GCTGCCCTATGTTTAGTC |
| rt-capK-r | ATGTTATTGATGCGTGTT |
| rt-capN-f | GACCTCGTTCAAAGATTA |
| rt-capN-r | CATAGTTGGTCGTAGGGT |
| rt-kdpA-f | ATTGTTCGGTTTATTGTCC |
| rt-kdpA-r | CATCATACTGCCCATTTCT |
| rt-kdpD-f | AGTCCAGGTGTTGGTAAA |
| rt-kdpD-r | TATTGTCGGCGATTCTTC |
| rt-kdpE-f | CTACAGCCGACAATGCCACA |
| rt-kdpE-r | TGCCCGAAGCTCATCAACA |
| rt-rna3-f | GGTTATTAAGTTGGGATGG |
| rt-rna3-r | GAGTGATTTCAATGGCACA |
| rt-agrA-f | AAAGTTGCAGCGATGGATTT |
| rt-agrA-r | ATGGGCAATGAGTCTGTGAG |
| rt-sarA-f | GACATACATCAGCGAAAA |
| rt-sarA-r | TACGTTGTTGTGCATTAA |
| rt-saeR-f | AAGTGGCGACCATTACAT |
| rt-saeR-r | CATTATTGCCTCAAATACGT |
| rt-mgrA-f | AGTACAATCTAACATACC |
| rt-mgrA-r | TTGCGATAAAGAAGAAGC |
| rt-ccpA-f | TCGTGGACTTGAAGATAT |
| rt-ccpA-r | CCATTTGTTCCTGATACT |
| rt-arlR-f | GCTGGGCTTGATTACGGT |
| rt-arlR-r | GCGCCATTTACCGTCACT |
| rt-sbcD-f | TTTTATACACTCCCTTATGC |
| rt-sbcD-r | GATGTCTTTCCACCTTGA |
| rt-spoVG-f | ACTCTGGCTTGTTCGTTG |
| rt-spoVG-r | CTTCTGATGTAGCGTTTT |
a
The sequences in lowercase letters refer to the restriction endonuclease recognition sites.