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. 2010 May 3;78(8):3323–3334. doi: 10.1128/IAI.00081-10

FIG. 5.

FIG. 5.

Confirmation of deletion of vapG. (A) PCR analysis of the vapG mutant. Primer pairs that specifically amplify regions across the deletion site were used in PCRs in which total DNA from R. equi 103+ (+) and the vapG mutant (Δ) served as the template. Standard DNA markers are indicated on the left (M). A no-template control (−) is also shown. (B) RT-PCR analysis of vapG expression. Total RNA was extracted from R. equi strains 103+ (+), the vapG mutant (Δ), and the complemented vapG mutant (Δ/G). cDNA was synthesized using equivalent concentrations of total RNA as the template. The presence of vapG and gyrB (internal control) was assessed by PCR using primer pairs specific for internal regions of each of the genes. Standard DNA markers are indicated on the left (M). No-template controls (−) and no-RT controls (NRT) are shown.