FIG. 2.

Three distinct populations of MHC class II-expressing cells in Toxoplasma-infected eyes. Mice were intravitreally injected with 10⁴ parasites, and 6 days later, eyes were harvested and analyzed by using flow cytometry. (A) FACS plot of CD11b and Ly6G expression on MHC class II⁺-gated cells. The distinct populations are highlighted as I (CD11b⁺ Ly6G⁺), II (CD11b⁺ Ly6G⁻), and III (CD11b⁻ Ly6G⁻). (B through D) Histograms of CD45 (B), Ly6C (C), and MHC class II (D) expression levels in each of the 3 populations. The dashed lines in panels B and C represent isotype control staining levels. MFI, mean fluorescence intensity of each population. Representative results from three independent experiments are shown.