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Inhibition of amastigote growth. After 48 h treatment with 7a (500 pmol·ml−1·well−1 concentration at 500 nM) or benznidazole (50 nmol·ml−1·well−1; 50 μM), infected cells were stained with Giemsa and analyzed. (A) Percentage of infected cells after treatment; (B) relative number of amastigotes per infected cell. *, P < 0.05.
