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Effects of compound 7a on parasite mitochondrion at 4°C or 37°C. DNA staining was performed with DAPI (blue) and the mitochondrion matrix marker with Alexa fluor 594 (red). (A) Nontreated trypomastigotes at 4°C; (B) 7a-treated trypomastigotes at 4°C; (C) nontreated trypomastigotes at 37°C; (D) 7a-treated trypomastigotes at 37°C.
