TABLE 1.
Strain | Genotype | MIC (MBC)a |
Etest valued | |||||
---|---|---|---|---|---|---|---|---|
LL-37 |
Em |
|||||||
Uninduced | Induced with LL-37b | Induced with Emc | Uninduced | Induced with LL-37b | Induced with Emc | |||
GA17457 | Wild type, parent | 500 (1,000) | 2,000 (>2,000) | 2,000 (>2,000) | 2 (16) | 8 (32) | 8 (>64) | 12 |
XZ8006 | mega::aad9 | 125 (250) | 125 (250) | N/A | 0.5 (4) | 0.5 (4) | N/A | 0.125 |
XZ8009 | mefE-mel::aph3 | 125 (250) | 64 (250) | N/A | 0.5 (4) | 0.125 (2) | N/A | 0.125 |
XZ7042 | bgaA::PmefE-lacZ | 500 (1,000) | 2,000 (>2,000) | 2,000 (>2,000) | 1 (8) | 16 (32) | 4 (>64) | 12 |
XZ8004 | mega::aad9 bgaA::PmefE-lacZ | 250 (500) | 125 (250) | N/A | 0.25 (4) | 0.5 (4) | N/A | 0.125 |
MICs and MBCs (μg/ml) determined by microdilution assays adapted to CAMPs (28). Em, erythromycin; N/A, not applicable because the strain was erythromycin sensitive.
Subinhibitory concentrations used for induction: GA17457 and XZ7042, 200 μg/ml; XZ8006 and XZ8009, 50 μg; and XZ8004, 100 μg.
Subinhibitory concentration used for induction: 1 μg/ml.
Etest was performed according to the manufacturer's instructions (AB Biodisk, Solna, Sweden).
Shown are the results obtained from one representative experiment, and at least three independent experiments were performed.