. 2010 May 24;54(8):3516–3519. doi: 10.1128/AAC.01756-09

TABLE 1.

MICs and MBCs of wild-type, mega, and mefE-mel mutant strains for LL-37 and erythromycin^e

Strain	Genotype	MIC (MBC)^a						Etest value^d
		LL-37			Em
		Uninduced	Induced with LL-37^b	Induced with Em^c	Uninduced	Induced with LL-37^b	Induced with Em^c
GA17457	Wild type, parent	500 (1,000)	2,000 (>2,000)	2,000 (>2,000)	2 (16)	8 (32)	8 (>64)	12
XZ8006	mega::aad9	125 (250)	125 (250)	N/A	0.5 (4)	0.5 (4)	N/A	0.125
XZ8009	mefE-mel::aph3	125 (250)	64 (250)	N/A	0.5 (4)	0.125 (2)	N/A	0.125
XZ7042	bgaA::PmefE-lacZ	500 (1,000)	2,000 (>2,000)	2,000 (>2,000)	1 (8)	16 (32)	4 (>64)	12
XZ8004	mega::aad9 bgaA::PmefE-lacZ	250 (500)	125 (250)	N/A	0.25 (4)	0.5 (4)	N/A	0.125

MICs and MBCs (μg/ml) determined by microdilution assays adapted to CAMPs (28). Em, erythromycin; N/A, not applicable because the strain was erythromycin sensitive.

Subinhibitory concentrations used for induction: GA17457 and XZ7042, 200 μg/ml; XZ8006 and XZ8009, 50 μg; and XZ8004, 100 μg.

Subinhibitory concentration used for induction: 1 μg/ml.

Etest was performed according to the manufacturer's instructions (AB Biodisk, Solna, Sweden).

Shown are the results obtained from one representative experiment, and at least three independent experiments were performed.