. 2010 Jun 1;54(8):3255–3261. doi: 10.1128/AAC.00397-10

TABLE 2.

Effect of human serum albumin and alpha-1-acid glycoprotein on EC₅₀s of NITD449 and NITD203

Assay condition^a	EC₅₀ (μM)
Assay condition^a	NITD449	NITD203
No HSA or AAG	2.61	0.69
40 mg/ml HSA	4.76	0.79
2 mg/ml AAG	2.52	0.76
40 mg/ml HSA and 2 mg/ml AAG	4.64	0.85

A549 cells were infected with DENV-2 (New Guinea C) at an MOI of 0.3 for 1 h without the compound. After removal of the viral inoculum, one set of the infected cells was incubated with medium containing various concentrations of NITD449 or NITD203, and another set of cells was incubated with medium containing the compound plus human serum albumin (HSA) and/or alpha-1-acid glycoprotein (AAG). On day 2 postinfection, viral titers in culture fluids were determined by a CFI assay. See Materials and Methods for details.