FIG. 2.

Impact of doxycycline repression of FKS1 on antifungal susceptibility in C. albicans biofilms. TET-FKS1-modulated biofilms were grown in 96-well plates for 6 h and treated for 24 h with serial dilutions of anidulafungin (A), flucytosine (B), or amphotericin B deoxycholate (C) in combination with doxycycline by using a checkerboard format. Endpoints were assessed using an XTT assay, and data are shown as percentages of biofilm growth relative to growth of untreated controls. Checkerboard assays were performed in duplicate, and results from one assay replicate are shown.